In this research, we visit literature directed seven steps procedure of scale development and incorporate it in studying dopamine, serotonin, epinephrine and norepinephrine and thus finalized 16 items neurotransmitters scale. We incorporated 6 samples for construction of reliable multi-aspect questionnaire that imitated across the samples. We confirm the content adequacy qualitatively and quantitatively including discriminant and convergent validity. We also established the criterion-related validity through the instrument’s relation with measures of behavioral aspects of individual investors. This research proposes that the neurotransmitters scale is valid and reliable. Neurotransmitters as dopamine, serotonin, epinephrine and norepinephrine have significant use for individual stock markets investors. This investigators hope that the corroborated scale is reliable as well as valid and will be appropriate to utilize in upcoming studies of neurofinance.