It was a comparative analysis of students' motivation in light of Glynn and Kobal-la (2006) five factors model at a higher level. Objectives were to assess the level of students' motivation and differences in the students' motivation on the basis of gender/age/university.The population was 9615 students of public universities of Islamabad. By using a convenient sampling technique, 260 students were selected. The tool was developed by Mubeen and Reid (2014), in light of the G AND K model. The data was analyzed by using Cronbach alpha reliability, intersection correlation, t-test, and ANOVA. The majority of respondents were above Average level of motivation. A significant difference between male and female students related to all the factors of motivation, and females were found better than male students. However, age-wise, no significant difference was found related to motivation. Overall, Air University students were found better than other universities. It is recommended that teachers and management need to plan group work for students not only involved but also engage them in positive competitions. There is a need to develop a sense of responsibility. Teachers need to set achievable goals as well for students to encourage task completion