The study was conducted specifically to examine the role of forensic experts and Sindh police in investigating various crimes in the Karachi and Hyderabad areas. Forensic science can use technical expertise to detect, identify and prosecute criminals. In Pakistan, the application and understanding of forensic science in law enforcement agencies is increasing. This research has been analyzed with the help of qualitative research methodology. When forensic experts were asked if forensic evidence in court is a comprehensive defense, it was found that about 93.3% has agreed on it, while 6.7% denied it. When police participants were asked if modern forensic techniques could give immediate results in any case, 58.8% agreed, while 41.2% refused. This signifies that 58.8% of police respondents have less forensic knowledge and acquaintance. The biggest obstacles are lack of funds and lack of trained personnel, equipment and laboratories. The future of forensic science in Sindh will depend on overcoming the barriers and strengthening capacity building and improvement.