The study was designed to compare science motivation and science achievement (Physics, Chemistry, and Biology) based on student gender and school location in Okara district. Using the cluster random sampling technique, 1000 students of 10th grade were selected as a sample of this study. An Urdu version of the Science Motivation Questionnaire was developed by the authors and used to collect data about student motivation towards science subjects, i.e., Physics, Chemistry, and Biology. The student achievement data were collected from their respective schools. The scale, which included five factors: self-efficacy, intrinsic motivation, self-determination, grade motivation, and career motivation demonstrated overall high-level reliability (α=.88) with factor-wise reliability ranging from .77 to .82. The study revealed that male students were significantly better than female students in Physics and Chemistry motivation, while female students were better in biology motivation. The recommendations have also been given in the study.